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fluoro ount g with dapi  (SouthernBiotech)


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    SouthernBiotech fluoro ount g with dapi
    Fig. 4. Cellular microarray data of cooperative influence of stiffness and ECM on HSC proliferative capacity. A.) Box and jitter plot illustrating average intracellular collagen I expression per cell per condition. Each colored dot is a unique ECM combination (34 per stiffness). B.) Heatmap of ECM effects on proliferation on 6 kPa substrates highlighting the large dynamic range of HSC proliferative capacity. C.) Bar plots of ECM combinations with highest and lowest average intracellular collagen I expression per stiffness, showcasing the impact of combinatorial ECM environments on collagen I expression D.) Ranked bar plot of all single and two ECM factor linear regression coefficients of HSC proliferation. Intercept coefficient = 25.32, Adjusted R2 = 0.037, F-statistic = 4.77 on 33 and 3200 degrees of freedom, p-value < 2.2e-16 E.) Representative images of single condition islands, with <t>DAPI</t> nuclei on the top row and EdU + nuclei on the bottom. From left to right: C1 + HA , C1 + LU, C3 + HA , and FN + LN. Scale bars are 500 μm and “ns” denotes p > 0.05, ∗for p < = 0.05, ∗∗for p < = 0.01, ∗∗∗for p < = 0.001, and ∗∗∗∗for p < = 0.0 0 01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Fluoro Ount G With Dapi, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 4320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluoro ount g with dapi/product/SouthernBiotech
    Average 96 stars, based on 4320 article reviews
    fluoro ount g with dapi - by Bioz Stars, 2026-04
    96/100 stars

    Images

    1) Product Images from "High throughput interrogation of human liver stellate cells reveals microenvironmental regulation of phenotype."

    Article Title: High throughput interrogation of human liver stellate cells reveals microenvironmental regulation of phenotype.

    Journal: Acta biomaterialia

    doi: 10.1016/j.actbio.2021.11.015

    Fig. 4. Cellular microarray data of cooperative influence of stiffness and ECM on HSC proliferative capacity. A.) Box and jitter plot illustrating average intracellular collagen I expression per cell per condition. Each colored dot is a unique ECM combination (34 per stiffness). B.) Heatmap of ECM effects on proliferation on 6 kPa substrates highlighting the large dynamic range of HSC proliferative capacity. C.) Bar plots of ECM combinations with highest and lowest average intracellular collagen I expression per stiffness, showcasing the impact of combinatorial ECM environments on collagen I expression D.) Ranked bar plot of all single and two ECM factor linear regression coefficients of HSC proliferation. Intercept coefficient = 25.32, Adjusted R2 = 0.037, F-statistic = 4.77 on 33 and 3200 degrees of freedom, p-value < 2.2e-16 E.) Representative images of single condition islands, with DAPI nuclei on the top row and EdU + nuclei on the bottom. From left to right: C1 + HA , C1 + LU, C3 + HA , and FN + LN. Scale bars are 500 μm and “ns” denotes p > 0.05, ∗for p < = 0.05, ∗∗for p < = 0.01, ∗∗∗for p < = 0.001, and ∗∗∗∗for p < = 0.0 0 01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Fig. 4. Cellular microarray data of cooperative influence of stiffness and ECM on HSC proliferative capacity. A.) Box and jitter plot illustrating average intracellular collagen I expression per cell per condition. Each colored dot is a unique ECM combination (34 per stiffness). B.) Heatmap of ECM effects on proliferation on 6 kPa substrates highlighting the large dynamic range of HSC proliferative capacity. C.) Bar plots of ECM combinations with highest and lowest average intracellular collagen I expression per stiffness, showcasing the impact of combinatorial ECM environments on collagen I expression D.) Ranked bar plot of all single and two ECM factor linear regression coefficients of HSC proliferation. Intercept coefficient = 25.32, Adjusted R2 = 0.037, F-statistic = 4.77 on 33 and 3200 degrees of freedom, p-value < 2.2e-16 E.) Representative images of single condition islands, with DAPI nuclei on the top row and EdU + nuclei on the bottom. From left to right: C1 + HA , C1 + LU, C3 + HA , and FN + LN. Scale bars are 500 μm and “ns” denotes p > 0.05, ∗for p < = 0.05, ∗∗for p < = 0.01, ∗∗∗for p < = 0.001, and ∗∗∗∗for p < = 0.0 0 01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Microarray, Expressing

    Fig. 7. Microarray platform analysis of the interplay between autophagy and cellular microenvironment on HSC phenotype. A.) Representative images of collagen I expression of HSCs cultured on C4 + FN on 1 kPa substrates responding to 72 h of bafilomycin treatment (top) versus control (bottom) B.) Bar plots of percent change in average HSC collagen I signal in response to bafilomycin treatment on 1 kPa substrates. C.) Bar plots of HSC proliferation response to 48, 24, and zero hours of bafilomycin on down-selected arrays. D.) Bar plots of HSC proliferation response to 24 h of bafilomycin treatment on 1 kPa substrate as a percent of control. E) Representative images of HSCs cultured on C3 + C4 on 1 kPa receiving 48 (right), 24 (middle), and zero hours (left) of bafilomycin treatment showcasing the impact of autophagy inhibition on HSC proliferation. DAPI images showing cell nuclei in blue are on top and EdU images showing Edu + nuclei in green are on bottom. Scale bars are 500 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Fig. 7. Microarray platform analysis of the interplay between autophagy and cellular microenvironment on HSC phenotype. A.) Representative images of collagen I expression of HSCs cultured on C4 + FN on 1 kPa substrates responding to 72 h of bafilomycin treatment (top) versus control (bottom) B.) Bar plots of percent change in average HSC collagen I signal in response to bafilomycin treatment on 1 kPa substrates. C.) Bar plots of HSC proliferation response to 48, 24, and zero hours of bafilomycin on down-selected arrays. D.) Bar plots of HSC proliferation response to 24 h of bafilomycin treatment on 1 kPa substrate as a percent of control. E) Representative images of HSCs cultured on C3 + C4 on 1 kPa receiving 48 (right), 24 (middle), and zero hours (left) of bafilomycin treatment showcasing the impact of autophagy inhibition on HSC proliferation. DAPI images showing cell nuclei in blue are on top and EdU images showing Edu + nuclei in green are on bottom. Scale bars are 500 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Microarray, Expressing, Cell Culture, Control, Inhibition



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    fluoro ount g with dapi  (SouthernBiotech)
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    SouthernBiotech fluoro ount g with dapi
    Fig. 4. Cellular microarray data of cooperative influence of stiffness and ECM on HSC proliferative capacity. A.) Box and jitter plot illustrating average intracellular collagen I expression per cell per condition. Each colored dot is a unique ECM combination (34 per stiffness). B.) Heatmap of ECM effects on proliferation on 6 kPa substrates highlighting the large dynamic range of HSC proliferative capacity. C.) Bar plots of ECM combinations with highest and lowest average intracellular collagen I expression per stiffness, showcasing the impact of combinatorial ECM environments on collagen I expression D.) Ranked bar plot of all single and two ECM factor linear regression coefficients of HSC proliferation. Intercept coefficient = 25.32, Adjusted R2 = 0.037, F-statistic = 4.77 on 33 and 3200 degrees of freedom, p-value < 2.2e-16 E.) Representative images of single condition islands, with <t>DAPI</t> nuclei on the top row and EdU + nuclei on the bottom. From left to right: C1 + HA , C1 + LU, C3 + HA , and FN + LN. Scale bars are 500 μm and “ns” denotes p > 0.05, ∗for p < = 0.05, ∗∗for p < = 0.01, ∗∗∗for p < = 0.001, and ∗∗∗∗for p < = 0.0 0 01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Fluoro Ount G With Dapi, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluoro ount g with dapi/product/SouthernBiotech
    Average 96 stars, based on 1 article reviews
    fluoro ount g with dapi - by Bioz Stars, 2026-04
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 4. Cellular microarray data of cooperative influence of stiffness and ECM on HSC proliferative capacity. A.) Box and jitter plot illustrating average intracellular collagen I expression per cell per condition. Each colored dot is a unique ECM combination (34 per stiffness). B.) Heatmap of ECM effects on proliferation on 6 kPa substrates highlighting the large dynamic range of HSC proliferative capacity. C.) Bar plots of ECM combinations with highest and lowest average intracellular collagen I expression per stiffness, showcasing the impact of combinatorial ECM environments on collagen I expression D.) Ranked bar plot of all single and two ECM factor linear regression coefficients of HSC proliferation. Intercept coefficient = 25.32, Adjusted R2 = 0.037, F-statistic = 4.77 on 33 and 3200 degrees of freedom, p-value < 2.2e-16 E.) Representative images of single condition islands, with DAPI nuclei on the top row and EdU + nuclei on the bottom. From left to right: C1 + HA , C1 + LU, C3 + HA , and FN + LN. Scale bars are 500 μm and “ns” denotes p > 0.05, ∗for p < = 0.05, ∗∗for p < = 0.01, ∗∗∗for p < = 0.001, and ∗∗∗∗for p < = 0.0 0 01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Acta biomaterialia

    Article Title: High throughput interrogation of human liver stellate cells reveals microenvironmental regulation of phenotype.

    doi: 10.1016/j.actbio.2021.11.015

    Figure Lengend Snippet: Fig. 4. Cellular microarray data of cooperative influence of stiffness and ECM on HSC proliferative capacity. A.) Box and jitter plot illustrating average intracellular collagen I expression per cell per condition. Each colored dot is a unique ECM combination (34 per stiffness). B.) Heatmap of ECM effects on proliferation on 6 kPa substrates highlighting the large dynamic range of HSC proliferative capacity. C.) Bar plots of ECM combinations with highest and lowest average intracellular collagen I expression per stiffness, showcasing the impact of combinatorial ECM environments on collagen I expression D.) Ranked bar plot of all single and two ECM factor linear regression coefficients of HSC proliferation. Intercept coefficient = 25.32, Adjusted R2 = 0.037, F-statistic = 4.77 on 33 and 3200 degrees of freedom, p-value < 2.2e-16 E.) Representative images of single condition islands, with DAPI nuclei on the top row and EdU + nuclei on the bottom. From left to right: C1 + HA , C1 + LU, C3 + HA , and FN + LN. Scale bars are 500 μm and “ns” denotes p > 0.05, ∗for p < = 0.05, ∗∗for p < = 0.01, ∗∗∗for p < = 0.001, and ∗∗∗∗for p < = 0.0 0 01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Finally, samples were mounted in Fluoro- ount G with DAPI (Southern Biotech, 0100–20) and imaged using n AxioScan Z.1 (Carl Zeiss, Inc) and associated Zen Pro-software o sooner than one day after mounting.

    Techniques: Microarray, Expressing

    Fig. 7. Microarray platform analysis of the interplay between autophagy and cellular microenvironment on HSC phenotype. A.) Representative images of collagen I expression of HSCs cultured on C4 + FN on 1 kPa substrates responding to 72 h of bafilomycin treatment (top) versus control (bottom) B.) Bar plots of percent change in average HSC collagen I signal in response to bafilomycin treatment on 1 kPa substrates. C.) Bar plots of HSC proliferation response to 48, 24, and zero hours of bafilomycin on down-selected arrays. D.) Bar plots of HSC proliferation response to 24 h of bafilomycin treatment on 1 kPa substrate as a percent of control. E) Representative images of HSCs cultured on C3 + C4 on 1 kPa receiving 48 (right), 24 (middle), and zero hours (left) of bafilomycin treatment showcasing the impact of autophagy inhibition on HSC proliferation. DAPI images showing cell nuclei in blue are on top and EdU images showing Edu + nuclei in green are on bottom. Scale bars are 500 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Acta biomaterialia

    Article Title: High throughput interrogation of human liver stellate cells reveals microenvironmental regulation of phenotype.

    doi: 10.1016/j.actbio.2021.11.015

    Figure Lengend Snippet: Fig. 7. Microarray platform analysis of the interplay between autophagy and cellular microenvironment on HSC phenotype. A.) Representative images of collagen I expression of HSCs cultured on C4 + FN on 1 kPa substrates responding to 72 h of bafilomycin treatment (top) versus control (bottom) B.) Bar plots of percent change in average HSC collagen I signal in response to bafilomycin treatment on 1 kPa substrates. C.) Bar plots of HSC proliferation response to 48, 24, and zero hours of bafilomycin on down-selected arrays. D.) Bar plots of HSC proliferation response to 24 h of bafilomycin treatment on 1 kPa substrate as a percent of control. E) Representative images of HSCs cultured on C3 + C4 on 1 kPa receiving 48 (right), 24 (middle), and zero hours (left) of bafilomycin treatment showcasing the impact of autophagy inhibition on HSC proliferation. DAPI images showing cell nuclei in blue are on top and EdU images showing Edu + nuclei in green are on bottom. Scale bars are 500 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Finally, samples were mounted in Fluoro- ount G with DAPI (Southern Biotech, 0100–20) and imaged using n AxioScan Z.1 (Carl Zeiss, Inc) and associated Zen Pro-software o sooner than one day after mounting.

    Techniques: Microarray, Expressing, Cell Culture, Control, Inhibition